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external standard kit λpolya  (TaKaRa)


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    Structured Review

    TaKaRa external standard kit λpolya
    External Standard Kit λpolya, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/external+standard+kit+%CE%BBpolya/External+Standard+Kit+(Lambda+PolyA)+for+qPCR/pm39753334-61-0-7
    Average 93 stars, based on 35 article reviews
    external standard kit λpolya - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: Gene Silencing Mediated by Endogenous MicroRNAs under Heat Stress Conditions in Mammalian Cells
    Article Snippet: The Perfect Real Time primers used were as follows (TAKARA BIO primer-set IDs): COL4A1 (HA156982), COL4A2 (HA174126), CABIN1 (HA183487), DNMT1 (HA002770), DICER1 (HA174786), LAMC1 (HA083467) and GAPDH (HA067812). .. The level of λpolyA + RNA-A as an external control was examined by qPCR using the AB 7300 Real Time PCR System (Applied Biosystems) with a FastStart Universal SYBR Green Master (Roche) and an External Standard Kit (λpolyA) for qPCR (TAKARA) according to the manufacturer's instructions. .. Cells were washed with PBS (Wako) and lysed in RIPA buffer [25 mM Tris-HCl (pH7.6), 0.1% SDS, 150 mM NaCl, 1% sodium deoxycholate, 0.5% NP-40] containing 1 x protease inhibitor cocktail (Protease Inhibitor Cocktail Tablets; Roche Diagnostics, Basel, Switzerland).

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study.
    Article Snippet: © Author(s) (or their employer(s)) 2025.. Reuse permitted under CC BYNC.. No commercial reuse.

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study
    Article Snippet: Complementary DNA was synthesised from the extracted RNA using the PrimeScript RT Reagent Kit (RR037A, TAKARA). .. External Standard Kit (λpolyA) for qPCR (3789, TAKARA) was added as an external control during the reverse transcription on the RNAs extracted from serum exosome samples. .. Quantitative PCR (qPCR) was conducted using GoTaq qPCR Master Mix (A6002, Promega) and a Fluorescent Quantitative PCR Instrument 480 (ROCHE).

    Real-time Polymerase Chain Reaction:

    Article Title: Gene Silencing Mediated by Endogenous MicroRNAs under Heat Stress Conditions in Mammalian Cells
    Article Snippet: The Perfect Real Time primers used were as follows (TAKARA BIO primer-set IDs): COL4A1 (HA156982), COL4A2 (HA174126), CABIN1 (HA183487), DNMT1 (HA002770), DICER1 (HA174786), LAMC1 (HA083467) and GAPDH (HA067812). .. The level of λpolyA + RNA-A as an external control was examined by qPCR using the AB 7300 Real Time PCR System (Applied Biosystems) with a FastStart Universal SYBR Green Master (Roche) and an External Standard Kit (λpolyA) for qPCR (TAKARA) according to the manufacturer's instructions. .. Cells were washed with PBS (Wako) and lysed in RIPA buffer [25 mM Tris-HCl (pH7.6), 0.1% SDS, 150 mM NaCl, 1% sodium deoxycholate, 0.5% NP-40] containing 1 x protease inhibitor cocktail (Protease Inhibitor Cocktail Tablets; Roche Diagnostics, Basel, Switzerland).

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study.
    Article Snippet: © Author(s) (or their employer(s)) 2025.. Reuse permitted under CC BYNC.. No commercial reuse.

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study
    Article Snippet: Complementary DNA was synthesised from the extracted RNA using the PrimeScript RT Reagent Kit (RR037A, TAKARA). .. External Standard Kit (λpolyA) for qPCR (3789, TAKARA) was added as an external control during the reverse transcription on the RNAs extracted from serum exosome samples. .. Quantitative PCR (qPCR) was conducted using GoTaq qPCR Master Mix (A6002, Promega) and a Fluorescent Quantitative PCR Instrument 480 (ROCHE).

    SYBR Green Assay:

    Article Title: Gene Silencing Mediated by Endogenous MicroRNAs under Heat Stress Conditions in Mammalian Cells
    Article Snippet: The Perfect Real Time primers used were as follows (TAKARA BIO primer-set IDs): COL4A1 (HA156982), COL4A2 (HA174126), CABIN1 (HA183487), DNMT1 (HA002770), DICER1 (HA174786), LAMC1 (HA083467) and GAPDH (HA067812). .. The level of λpolyA + RNA-A as an external control was examined by qPCR using the AB 7300 Real Time PCR System (Applied Biosystems) with a FastStart Universal SYBR Green Master (Roche) and an External Standard Kit (λpolyA) for qPCR (TAKARA) according to the manufacturer's instructions. .. Cells were washed with PBS (Wako) and lysed in RIPA buffer [25 mM Tris-HCl (pH7.6), 0.1% SDS, 150 mM NaCl, 1% sodium deoxycholate, 0.5% NP-40] containing 1 x protease inhibitor cocktail (Protease Inhibitor Cocktail Tablets; Roche Diagnostics, Basel, Switzerland).

    Reverse Transcription:

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study.
    Article Snippet: © Author(s) (or their employer(s)) 2025.. Reuse permitted under CC BYNC.. No commercial reuse.

    Article Title: Construction of exosome non-coding RNA feature for non-invasive, early detection of gastric cancer patients by machine learning: a multi-cohort study
    Article Snippet: Complementary DNA was synthesised from the extracted RNA using the PrimeScript RT Reagent Kit (RR037A, TAKARA). .. External Standard Kit (λpolyA) for qPCR (3789, TAKARA) was added as an external control during the reverse transcription on the RNAs extracted from serum exosome samples. .. Quantitative PCR (qPCR) was conducted using GoTaq qPCR Master Mix (A6002, Promega) and a Fluorescent Quantitative PCR Instrument 480 (ROCHE).



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